Construct: shRNA TRCN0000281605

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001099777.2-399s21c1
DNA Barcode:
CGACATGAACACGGATGTAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SLC66A1L (152078)
Gene Description:
solute carrier family 66 member 1 like
Transcript:
RefSeq NM_001099777.2 (NON-CURRENT)
Match location:
Position 399 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000281605)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGACATGAACACGGATGTAAT
Hairpin Sequence:
5'-CCGG-CGACATGAACACGGATGTAAT-CTCGAG-ATTACATCCGTGTTCATGTCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGACATGAACACGGATGTAATCTCGAGATTACATCCGTGTTCATGTCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGACATGAACACGGATGTAATCTCGAGATTACATCCGTGTTCATGTCG-3'

Other clones with same target sequence:

(none)