Construct: shRNA TRCN0000281619

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_028834.2-1261s21c1
DNA Barcode:
GAGCGACTCAGCTGCAATTAC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Slx4ip (74243)
Gene Description:
SLX4 interacting protein
Transcript:
RefSeq NM_028834.2 (CURRENT)
Match location:
Position 1261 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000281619)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGCGACTCAGCTGCAATTAC
Hairpin Sequence:
5'-CCGG-GAGCGACTCAGCTGCAATTAC-CTCGAG-GTAATTGCAGCTGAGTCGCTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGCGACTCAGCTGCAATTACCTCGAGGTAATTGCAGCTGAGTCGCTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGCGACTCAGCTGCAATTACCTCGAGGTAATTGCAGCTGAGTCGCTC-3'

Other clones with same target sequence:

(none)