Construct: shRNA TRCN0000281649

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_022018.2-848s21c1
DNA Barcode:
CCAAGTTTCAGAAGGATTAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Fam129a (63913)
Gene Description:
family with sequence similarity 129, member A
Transcript:
RefSeq NM_022018.2 (NON-CURRENT)
Match location:
Position 848 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000281649)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAAGTTTCAGAAGGATTAAA
Hairpin Sequence:
5'-CCGG-CCAAGTTTCAGAAGGATTAAA-CTCGAG-TTTAATCCTTCTGAAACTTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAAGTTTCAGAAGGATTAAACTCGAGTTTAATCCTTCTGAAACTTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAAGTTTCAGAAGGATTAAACTCGAGTTTAATCCTTCTGAAACTTGG-3'

Other clones with same target sequence:

(none)