Construct: shRNA TRCN0000281934

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001113806.1-2086s21c1
DNA Barcode:
ATCATGAAAGAGCAAGTATAG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gm14410 (100043403)
Gene Description:
predicted gene 14410
Transcript:
RefSeq NM_001113806.1 (NON-CURRENT)
Match location:
Position 2086 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000281934)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATCATGAAAGAGCAAGTATAG
Hairpin Sequence:
5'-CCGG-ATCATGAAAGAGCAAGTATAG-CTCGAG-CTATACTTGCTCTTTCATGAT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATCATGAAAGAGCAAGTATAGCTCGAGCTATACTTGCTCTTTCATGATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATCATGAAAGAGCAAGTATAGCTCGAGCTATACTTGCTCTTTCATGAT-3'

Other clones with same target sequence:

(none)