Construct: shRNA TRCN0000281978

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_145804.1-2914s21c1
DNA Barcode:
GCCCTAGAGCTGTTTGTTCAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ABTB2 (25841)
Gene Description:
ankyrin repeat and BTB domain containing 2
Transcript:
RefSeq NM_145804.1 (NON-CURRENT)
Match location:
Position 2914 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000281978)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCCTAGAGCTGTTTGTTCAA
Hairpin Sequence:
5'-CCGG-GCCCTAGAGCTGTTTGTTCAA-CTCGAG-TTGAACAAACAGCTCTAGGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCCTAGAGCTGTTTGTTCAACTCGAGTTGAACAAACAGCTCTAGGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCCTAGAGCTGTTTGTTCAACTCGAGTTGAACAAACAGCTCTAGGGC-3'

Other clones with same target sequence:

  1. TRCN0000138256