Construct: shRNA TRCN0000281992

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_145358.1-2028s21c1
DNA Barcode:
GACTCCATGTCGTCGACTTTG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Camkk2 (207565)
Gene Description:
calcium/calmodulin-dependent protein kinase kinase 2, beta
Transcript:
RefSeq NM_145358.1 (NON-CURRENT)
Match location:
Position 2028 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000281992)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GACTCCATGTCGTCGACTTTG
Hairpin Sequence:
5'-CCGG-GACTCCATGTCGTCGACTTTG-CTCGAG-CAAAGTCGACGACATGGAGTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGACTCCATGTCGTCGACTTTGCTCGAGCAAAGTCGACGACATGGAGTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGACTCCATGTCGTCGACTTTGCTCGAGCAAAGTCGACGACATGGAGTC-3'

Other clones with same target sequence:

(none)