Construct: shRNA TRCN0000282025

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_017787.4-1049s21c1
DNA Barcode:
CGATGACCTCAAAGAGTTCAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
WBP1L (54838)
Gene Description:
WW domain binding protein 1 like
Transcript:
RefSeq NM_017787.4 (NON-CURRENT)
Match location:
Position 1049 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000282025)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGATGACCTCAAAGAGTTCAA
Hairpin Sequence:
5'-CCGG-CGATGACCTCAAAGAGTTCAA-CTCGAG-TTGAACTCTTTGAGGTCATCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGATGACCTCAAAGAGTTCAACTCGAGTTGAACTCTTTGAGGTCATCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGATGACCTCAAAGAGTTCAACTCGAGTTGAACTCTTTGAGGTCATCG-3'

Other clones with same target sequence:

  1. TRCN0000135803