Construct: shRNA TRCN0000282104

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001045513.2-885s21c1
DNA Barcode:
ACGCCCTCAAGAGCTTGATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Raph1 (77300)
Gene Description:
Ras association (RalGDS/AF-6) and pleckstrin homology domains 1
Transcript:
RefSeq NM_001045513.2 (NON-CURRENT)
Match location:
Position 885 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000282104)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACGCCCTCAAGAGCTTGATTT
Hairpin Sequence:
5'-CCGG-ACGCCCTCAAGAGCTTGATTT-CTCGAG-AAATCAAGCTCTTGAGGGCGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACGCCCTCAAGAGCTTGATTTCTCGAGAAATCAAGCTCTTGAGGGCGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACGCCCTCAAGAGCTTGATTTCTCGAGAAATCAAGCTCTTGAGGGCGT-3'

Other clones with same target sequence:

(none)