Construct: shRNA TRCN0000282235

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001078170.1-3318s21c1
DNA Barcode:
TAGTTGAACATCGACATCAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RGPD2 (729857)
Gene Description:
RANBP2 like and GRIP domain containing 2
Transcript:
RefSeq NM_001078170.1 (NON-CURRENT)
Match location:
Position 3318 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000282235)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TAGTTGAACATCGACATCAAA
Hairpin Sequence:
5'-CCGG-TAGTTGAACATCGACATCAAA-CTCGAG-TTTGATGTCGATGTTCAACTA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTAGTTGAACATCGACATCAAACTCGAGTTTGATGTCGATGTTCAACTATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATAGTTGAACATCGACATCAAACTCGAGTTTGATGTCGATGTTCAACTA-3'

Other clones with same target sequence:

(none)