Construct: shRNA TRCN0000282305

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007489.3-953s21c1
DNA Barcode:
GACGAACTGAAACACCTAATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Arntl (11865)
Gene Description:
aryl hydrocarbon receptor nuclear translocator-like
Transcript:
RefSeq NM_007489.3 (NON-CURRENT)
Match location:
Position 953 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000282305)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GACGAACTGAAACACCTAATT
Hairpin Sequence:
5'-CCGG-GACGAACTGAAACACCTAATT-CTCGAG-AATTAGGTGTTTCAGTTCGTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGACGAACTGAAACACCTAATTCTCGAGAATTAGGTGTTTCAGTTCGTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGACGAACTGAAACACCTAATTCTCGAGAATTAGGTGTTTCAGTTCGTC-3'

Other clones with same target sequence:

(none)