Construct: shRNA TRCN0000282384

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015764.3-5374s21c1
DNA Barcode:
TCTGGCCCTTCATCGTCATTG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Greb1 (268527)
Gene Description:
gene regulated by estrogen in breast cancer protein
Transcript:
RefSeq NM_015764.3 (NON-CURRENT)
Match location:
Position 5374 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000282384)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCTGGCCCTTCATCGTCATTG
Hairpin Sequence:
5'-CCGG-TCTGGCCCTTCATCGTCATTG-CTCGAG-CAATGACGATGAAGGGCCAGA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCTGGCCCTTCATCGTCATTGCTCGAGCAATGACGATGAAGGGCCAGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCTGGCCCTTCATCGTCATTGCTCGAGCAATGACGATGAAGGGCCAGA-3'

Other clones with same target sequence:

(none)