Construct: shRNA TRCN0000282520

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007886.1-447s21c1
DNA Barcode:
CATCAGTCTCCTACTCAATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Dtnb (13528)
Gene Description:
dystrobrevin, beta
Transcript:
RefSeq NM_007886.1 (NON-CURRENT)
Match location:
Position 447 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000282520)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CATCAGTCTCCTACTCAATTT
Hairpin Sequence:
5'-CCGG-CATCAGTCTCCTACTCAATTT-CTCGAG-AAATTGAGTAGGAGACTGATG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCATCAGTCTCCTACTCAATTTCTCGAGAAATTGAGTAGGAGACTGATGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACATCAGTCTCCTACTCAATTTCTCGAGAAATTGAGTAGGAGACTGATG-3'

Other clones with same target sequence:

(none)