Construct: shRNA TRCN0000282524

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001123371.1-1002s21c1
DNA Barcode:
GATGTGCTGCTTCTCTAATTC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Pnp2 (667034)
Gene Description:
purine-nucleoside phosphorylase 2
Transcript:
RefSeq NM_001123371.1 (NON-CURRENT)
Match location:
Position 1002 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000282524)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATGTGCTGCTTCTCTAATTC
Hairpin Sequence:
5'-CCGG-GATGTGCTGCTTCTCTAATTC-CTCGAG-GAATTAGAGAAGCAGCACATC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATGTGCTGCTTCTCTAATTCCTCGAGGAATTAGAGAAGCAGCACATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATGTGCTGCTTCTCTAATTCCTCGAGGAATTAGAGAAGCAGCACATC-3'

Other clones with same target sequence:

(none)