Construct: shRNA TRCN0000282533

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001127725.1-1109s21c1
DNA Barcode:
TCTGCTGCAGCACGTTCTTTC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Sec14l5 (665119)
Gene Description:
SEC14-like lipid binding 5
Transcript:
RefSeq NM_001127725.1 (CURRENT)
Match location:
Position 1109 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000282533)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCTGCTGCAGCACGTTCTTTC
Hairpin Sequence:
5'-CCGG-TCTGCTGCAGCACGTTCTTTC-CTCGAG-GAAAGAACGTGCTGCAGCAGA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCTGCTGCAGCACGTTCTTTCCTCGAGGAAAGAACGTGCTGCAGCAGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCTGCTGCAGCACGTTCTTTCCTCGAGGAAAGAACGTGCTGCAGCAGA-3'

Other clones with same target sequence:

(none)