Construct: shRNA TRCN0000282560

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_020854.3-1085s21c1
DNA Barcode:
ATGGGATGATGTAGGATTAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RELCH (57614)
Gene Description:
RAB11 binding and LisH domain, coiled-coil and HEAT repeat containing
Transcript:
RefSeq NM_020854.3 (NON-CURRENT)
Match location:
Position 1085 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000282560)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATGGGATGATGTAGGATTAAA
Hairpin Sequence:
5'-CCGG-ATGGGATGATGTAGGATTAAA-CTCGAG-TTTAATCCTACATCATCCCAT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATGGGATGATGTAGGATTAAACTCGAGTTTAATCCTACATCATCCCATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATGGGATGATGTAGGATTAAACTCGAGTTTAATCCTACATCATCCCAT-3'

Other clones with same target sequence:

(none)