Construct: shRNA TRCN0000282735

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_021184.3-1164s21c1
DNA Barcode:
CAAACTCAGATGGGATCATGT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
C6orf47 (57827)
Gene Description:
chromosome 6 open reading frame 47
Transcript:
RefSeq NM_021184.3 (NON-CURRENT)
Match location:
Position 1164 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000282735)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAAACTCAGATGGGATCATGT
Hairpin Sequence:
5'-CCGG-CAAACTCAGATGGGATCATGT-CTCGAG-ACATGATCCCATCTGAGTTTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAAACTCAGATGGGATCATGTCTCGAGACATGATCCCATCTGAGTTTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAAACTCAGATGGGATCATGTCTCGAGACATGATCCCATCTGAGTTTG-3'

Other clones with same target sequence:

(none)