Construct: shRNA TRCN0000282791
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_152769.2-325s21c1
- DNA Barcode:
- TGACGACGTCGTGGGACAATG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- CBARP (255057)
- Gene Description:
- CACN subunit beta associated regulatory protein
- Transcript:
- RefSeq NM_152769.2 (NON-CURRENT)
- Match location:
- Position 325 (CDS)
Vector Information:
- Vector Backbone:
- pLKO_005
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000282791)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- TGACGACGTCGTGGGACAATG
- Hairpin Sequence:
-
5'-CCGG-TGACGACGTCGTGGGACAATG-CTCGAG-CATTGTCCCACGACGTCGTCA-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGTGACGACGTCGTGGGACAATGCTCGAGCATTGTCCCACGACGTCGTCATTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAATGACGACGTCGTGGGACAATGCTCGAGCATTGTCCCACGACGTCGTCA-3'
Other clones with same target sequence:
(none)
