Construct: shRNA TRCN0000282857

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_017609.2-1604s21c1
DNA Barcode:
GTTCGATGAAGGGACAATTTC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CFAP46 (54777)
Gene Description:
cilia and flagella associated protein 46
Transcript:
RefSeq NM_017609.2 (NON-CURRENT)
Match location:
Position 1604 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000282857)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTTCGATGAAGGGACAATTTC
Hairpin Sequence:
5'-CCGG-GTTCGATGAAGGGACAATTTC-CTCGAG-GAAATTGTCCCTTCATCGAAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTTCGATGAAGGGACAATTTCCTCGAGGAAATTGTCCCTTCATCGAACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTTCGATGAAGGGACAATTTCCTCGAGGAAATTGTCCCTTCATCGAAC-3'

Other clones with same target sequence:

(none)