Construct: shRNA TRCN0000282861

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_152335.2-2205s21c1
DNA Barcode:
CCATCTGAGCCTATGTCATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TMEM266 (123591)
Gene Description:
transmembrane protein 266
Transcript:
RefSeq NM_152335.2 (NON-CURRENT)
Match location:
Position 2205 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000282861)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCATCTGAGCCTATGTCATTT
Hairpin Sequence:
5'-CCGG-CCATCTGAGCCTATGTCATTT-CTCGAG-AAATGACATAGGCTCAGATGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCATCTGAGCCTATGTCATTTCTCGAGAAATGACATAGGCTCAGATGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCATCTGAGCCTATGTCATTTCTCGAGAAATGACATAGGCTCAGATGG-3'

Other clones with same target sequence:

(none)