Construct: shRNA TRCN0000282909

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001085447.1-1621s21c1
DNA Barcode:
TGTGGACAGAGGTGGATTAAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CCDC173 (129881)
Gene Description:
coiled-coil domain containing 173
Transcript:
RefSeq NM_001085447.1 (NON-CURRENT)
Match location:
Position 1621 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000282909)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGTGGACAGAGGTGGATTAAG
Hairpin Sequence:
5'-CCGG-TGTGGACAGAGGTGGATTAAG-CTCGAG-CTTAATCCACCTCTGTCCACA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGTGGACAGAGGTGGATTAAGCTCGAGCTTAATCCACCTCTGTCCACATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGTGGACAGAGGTGGATTAAGCTCGAGCTTAATCCACCTCTGTCCACA-3'

Other clones with same target sequence:

(none)