Construct: shRNA TRCN0000282951

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_028731.5-1688s21c1
DNA Barcode:
GACCTTGAGGTTGAGGTTAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Esyt2 (52635)
Gene Description:
extended synaptotagmin-like protein 2
Transcript:
RefSeq NM_028731.5 (CURRENT)
Match location:
Position 1688 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000282951)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GACCTTGAGGTTGAGGTTAAA
Hairpin Sequence:
5'-CCGG-GACCTTGAGGTTGAGGTTAAA-CTCGAG-TTTAACCTCAACCTCAAGGTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGACCTTGAGGTTGAGGTTAAACTCGAGTTTAACCTCAACCTCAAGGTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGACCTTGAGGTTGAGGTTAAACTCGAGTTTAACCTCAACCTCAAGGTC-3'

Other clones with same target sequence:

(none)