Construct: shRNA TRCN0000283170

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_026062.3-746s21c1
DNA Barcode:
ATAAGTCTACCATGGGTTATG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Fam69a (67266)
Gene Description:
family with sequence similarity 69, member A
Transcript:
RefSeq NM_026062.3 (NON-CURRENT)
Match location:
Position 746 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000283170)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATAAGTCTACCATGGGTTATG
Hairpin Sequence:
5'-CCGG-ATAAGTCTACCATGGGTTATG-CTCGAG-CATAACCCATGGTAGACTTAT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATAAGTCTACCATGGGTTATGCTCGAGCATAACCCATGGTAGACTTATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATAAGTCTACCATGGGTTATGCTCGAGCATAACCCATGGTAGACTTAT-3'

Other clones with same target sequence:

(none)