Construct: shRNA TRCN0000283337

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_019772.2-624s21c1
DNA Barcode:
TCCTAACTACCAAACACTTAG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
1110004F10Rik (56372)
Gene Description:
RIKEN cDNA 1110004F10 gene
Transcript:
RefSeq NM_019772.2 (CURRENT)
Match location:
Position 624 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000283337)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCCTAACTACCAAACACTTAG
Hairpin Sequence:
5'-CCGG-TCCTAACTACCAAACACTTAG-CTCGAG-CTAAGTGTTTGGTAGTTAGGA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCCTAACTACCAAACACTTAGCTCGAGCTAAGTGTTTGGTAGTTAGGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCCTAACTACCAAACACTTAGCTCGAGCTAAGTGTTTGGTAGTTAGGA-3'

Other clones with same target sequence:

(none)