Construct: shRNA TRCN0000283408

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001085493.1-521s21c1
DNA Barcode:
GACAAAGCGTACCGCAGTATG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ndufaf6 (76947)
Gene Description:
NADH dehydrogenase (ubiquinone) complex I, assembly factor 6
Transcript:
RefSeq NM_001085493.1 (NON-CURRENT)
Match location:
Position 521 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000283408)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GACAAAGCGTACCGCAGTATG
Hairpin Sequence:
5'-CCGG-GACAAAGCGTACCGCAGTATG-CTCGAG-CATACTGCGGTACGCTTTGTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGACAAAGCGTACCGCAGTATGCTCGAGCATACTGCGGTACGCTTTGTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGACAAAGCGTACCGCAGTATGCTCGAGCATACTGCGGTACGCTTTGTC-3'

Other clones with same target sequence:

(none)