Construct: shRNA TRCN0000283618

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_027860.2-927s21c1
DNA Barcode:
GATTGTGAGATTCCCGCTTTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
0610010F05Rik (71675)
Gene Description:
RIKEN cDNA 0610010F05 gene
Transcript:
RefSeq NM_027860.2 (NON-CURRENT)
Match location:
Position 927 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000283618)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATTGTGAGATTCCCGCTTTA
Hairpin Sequence:
5'-CCGG-GATTGTGAGATTCCCGCTTTA-CTCGAG-TAAAGCGGGAATCTCACAATC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATTGTGAGATTCCCGCTTTACTCGAGTAAAGCGGGAATCTCACAATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATTGTGAGATTCCCGCTTTACTCGAGTAAAGCGGGAATCTCACAATC-3'

Other clones with same target sequence:

(none)