Construct: shRNA TRCN0000283758

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001080429.2-3613s21c1
DNA Barcode:
ATCGCATTCTGGAGGAAATTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CAMSAP3 (57662)
Gene Description:
calmodulin regulated spectrin associated protein family member 3
Transcript:
RefSeq NM_001080429.2 (NON-CURRENT)
Match location:
Position 3613 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000283758)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATCGCATTCTGGAGGAAATTG
Hairpin Sequence:
5'-CCGG-ATCGCATTCTGGAGGAAATTG-CTCGAG-CAATTTCCTCCAGAATGCGAT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATCGCATTCTGGAGGAAATTGCTCGAGCAATTTCCTCCAGAATGCGATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATCGCATTCTGGAGGAAATTGCTCGAGCAATTTCCTCCAGAATGCGAT-3'

Other clones with same target sequence:

(none)