Construct: shRNA TRCN0000283760

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_020722.1-1722s21c1
DNA Barcode:
ACTCCGAGGAGCCAGGTATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CRACD (57482)
Gene Description:
capping protein inhibiting regulator of actin dynamics
Transcript:
RefSeq NM_020722.1 (CURRENT)
Match location:
Position 1722 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000283760)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACTCCGAGGAGCCAGGTATTT
Hairpin Sequence:
5'-CCGG-ACTCCGAGGAGCCAGGTATTT-CTCGAG-AAATACCTGGCTCCTCGGAGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACTCCGAGGAGCCAGGTATTTCTCGAGAAATACCTGGCTCCTCGGAGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACTCCGAGGAGCCAGGTATTTCTCGAGAAATACCTGGCTCCTCGGAGT-3'

Other clones with same target sequence:

(none)