Construct: shRNA TRCN0000283785

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_175518.5-636s21c1
DNA Barcode:
TGCAAGCACTCAACGGTATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tmem245 (242474)
Gene Description:
transmembrane protein 245
Transcript:
RefSeq NM_175518.5 (CURRENT)
Match location:
Position 636 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000283785)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGCAAGCACTCAACGGTATTT
Hairpin Sequence:
5'-CCGG-TGCAAGCACTCAACGGTATTT-CTCGAG-AAATACCGTTGAGTGCTTGCA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGCAAGCACTCAACGGTATTTCTCGAGAAATACCGTTGAGTGCTTGCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGCAAGCACTCAACGGTATTTCTCGAGAAATACCGTTGAGTGCTTGCA-3'

Other clones with same target sequence:

(none)