Construct: shRNA TRCN0000283825

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001001881.1-690s21c1
DNA Barcode:
TCTGCACACGATGCCTTAATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
2510009E07Rik (72190)
Gene Description:
RIKEN cDNA 2510009E07 gene
Transcript:
RefSeq NM_001001881.1 (NON-CURRENT)
Match location:
Position 690 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000283825)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCTGCACACGATGCCTTAATT
Hairpin Sequence:
5'-CCGG-TCTGCACACGATGCCTTAATT-CTCGAG-AATTAAGGCATCGTGTGCAGA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCTGCACACGATGCCTTAATTCTCGAGAATTAAGGCATCGTGTGCAGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCTGCACACGATGCCTTAATTCTCGAGAATTAAGGCATCGTGTGCAGA-3'

Other clones with same target sequence:

  1. TRCN0000264112