Construct: shRNA TRCN0000283903

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_177674.4-1948s21c1
DNA Barcode:
CTACTTACACAATCAACTATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cfap74 (544678)
Gene Description:
cilia and flagella associated protein 74
Transcript:
RefSeq NM_177674.4 (NON-CURRENT)
Match location:
Position 1948 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000283903)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTACTTACACAATCAACTATT
Hairpin Sequence:
5'-CCGG-CTACTTACACAATCAACTATT-CTCGAG-AATAGTTGATTGTGTAAGTAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTACTTACACAATCAACTATTCTCGAGAATAGTTGATTGTGTAAGTAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTACTTACACAATCAACTATTCTCGAGAATAGTTGATTGTGTAAGTAG-3'

Other clones with same target sequence:

(none)