Construct: shRNA TRCN0000284123

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001129979.1-58s21c1
DNA Barcode:
GAAGGGCAAGCCAAGTCTTTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SYCE1L (100130958)
Gene Description:
synaptonemal complex central element protein 1 like
Transcript:
RefSeq NM_001129979.1 (NON-CURRENT)
Match location:
Position 58 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000284123)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAAGGGCAAGCCAAGTCTTTG
Hairpin Sequence:
5'-CCGG-GAAGGGCAAGCCAAGTCTTTG-CTCGAG-CAAAGACTTGGCTTGCCCTTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAAGGGCAAGCCAAGTCTTTGCTCGAGCAAAGACTTGGCTTGCCCTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAAGGGCAAGCCAAGTCTTTGCTCGAGCAAAGACTTGGCTTGCCCTTC-3'

Other clones with same target sequence:

(none)