Construct: shRNA TRCN0000284144

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001137556.1-150s21c1
DNA Barcode:
ACTGGAGAGAAAGCCATTGCT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
FAM25BP (100132929)
Gene Description:
family with sequence similarity 25 member B, pseudogene; protein FAM25
Transcript:
RefSeq NM_001137556.1 (NON-CURRENT)
Match location:
Position 150 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000284144)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACTGGAGAGAAAGCCATTGCT
Hairpin Sequence:
5'-CCGG-ACTGGAGAGAAAGCCATTGCT-CTCGAG-AGCAATGGCTTTCTCTCCAGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACTGGAGAGAAAGCCATTGCTCTCGAGAGCAATGGCTTTCTCTCCAGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACTGGAGAGAAAGCCATTGCTCTCGAGAGCAATGGCTTTCTCTCCAGT-3'

Other clones with same target sequence:

(none)