Construct: shRNA TRCN0000284235

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001034880.2-1303s21c1
DNA Barcode:
TTGTACTTGCATGCCAAATAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gm5460 (432838)
Gene Description:
predicted gene 5460
Transcript:
RefSeq NM_001034880.2 (CURRENT)
Match location:
Position 1303 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000284235)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TTGTACTTGCATGCCAAATAT
Hairpin Sequence:
5'-CCGG-TTGTACTTGCATGCCAAATAT-CTCGAG-ATATTTGGCATGCAAGTACAA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTTGTACTTGCATGCCAAATATCTCGAGATATTTGGCATGCAAGTACAATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATTGTACTTGCATGCCAAATATCTCGAGATATTTGGCATGCAAGTACAA-3'

Other clones with same target sequence:

(none)