Construct: shRNA TRCN0000284272

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001101546.1-133s21c1
DNA Barcode:
GCCCATGTCTCAGTATGCTTC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tmem233 (545798)
Gene Description:
transmembrane protein 233
Transcript:
RefSeq NM_001101546.1 (CURRENT)
Match location:
Position 133 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000284272)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCCATGTCTCAGTATGCTTC
Hairpin Sequence:
5'-CCGG-GCCCATGTCTCAGTATGCTTC-CTCGAG-GAAGCATACTGAGACATGGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCCATGTCTCAGTATGCTTCCTCGAGGAAGCATACTGAGACATGGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCCATGTCTCAGTATGCTTCCTCGAGGAAGCATACTGAGACATGGGC-3'

Other clones with same target sequence:

(none)