Construct: shRNA TRCN0000284338

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011361.1-1146s21c1
DNA Barcode:
TGGGATGATCTCATCAATAAG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Sgk1 (20393)
Gene Description:
serum/glucocorticoid regulated kinase 1
Transcript:
RefSeq NM_011361.1 (NON-CURRENT)
Match location:
Position 1146 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000284338)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGGGATGATCTCATCAATAAG
Hairpin Sequence:
5'-CCGG-TGGGATGATCTCATCAATAAG-CTCGAG-CTTATTGATGAGATCATCCCA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGGGATGATCTCATCAATAAGCTCGAGCTTATTGATGAGATCATCCCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGGGATGATCTCATCAATAAGCTCGAGCTTATTGATGAGATCATCCCA-3'

Other clones with same target sequence:

(none)