Construct: shRNA TRCN0000284560

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_027405.2-849s21c1
DNA Barcode:
CCCGGATTCACTGTCCATAAC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gpatch2l (70373)
Gene Description:
G patch domain containing 2 like
Transcript:
RefSeq NM_027405.2 (NON-CURRENT)
Match location:
Position 849 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000284560)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCGGATTCACTGTCCATAAC
Hairpin Sequence:
5'-CCGG-CCCGGATTCACTGTCCATAAC-CTCGAG-GTTATGGACAGTGAATCCGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCGGATTCACTGTCCATAACCTCGAGGTTATGGACAGTGAATCCGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCGGATTCACTGTCCATAACCTCGAGGTTATGGACAGTGAATCCGGG-3'

Other clones with same target sequence:

(none)