Construct: shRNA TRCN0000284640

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001113736.1-114s21c1
DNA Barcode:
TACAGAGCCTGGCGAAGAATG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gm13040 (100040854)
Gene Description:
predicted gene 13040
Transcript:
RefSeq NM_001113736.1 (CURRENT)
Match location:
Position 114 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000284640)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TACAGAGCCTGGCGAAGAATG
Hairpin Sequence:
5'-CCGG-TACAGAGCCTGGCGAAGAATG-CTCGAG-CATTCTTCGCCAGGCTCTGTA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTACAGAGCCTGGCGAAGAATGCTCGAGCATTCTTCGCCAGGCTCTGTATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATACAGAGCCTGGCGAAGAATGCTCGAGCATTCTTCGCCAGGCTCTGTA-3'

Other clones with same target sequence:

(none)