Construct: shRNA TRCN0000284688

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001099919.1-661s21c1
DNA Barcode:
GAAGTCCATGGAGGGTTTGAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gm10147 (100039324)
Gene Description:
predicted gene 10147
Transcript:
RefSeq NM_001099919.1 (NON-CURRENT)
Match location:
Position 661 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000284688)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAAGTCCATGGAGGGTTTGAT
Hairpin Sequence:
5'-CCGG-GAAGTCCATGGAGGGTTTGAT-CTCGAG-ATCAAACCCTCCATGGACTTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAAGTCCATGGAGGGTTTGATCTCGAGATCAAACCCTCCATGGACTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAAGTCCATGGAGGGTTTGATCTCGAGATCAAACCCTCCATGGACTTC-3'

Other clones with same target sequence:

(none)