Construct: shRNA TRCN0000284761

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001955.3-1429s21c1
DNA Barcode:
AGACAAACATGCAAGTAAAGA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
EDN1 (1906)
Gene Description:
endothelin 1
Transcript:
RefSeq NM_001955.3 (NON-CURRENT)
Match location:
Position 1429 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000284761)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGACAAACATGCAAGTAAAGA
Hairpin Sequence:
5'-CCGG-AGACAAACATGCAAGTAAAGA-CTCGAG-TCTTTACTTGCATGTTTGTCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGACAAACATGCAAGTAAAGACTCGAGTCTTTACTTGCATGTTTGTCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGACAAACATGCAAGTAAAGACTCGAGTCTTTACTTGCATGTTTGTCT-3'

Other clones with same target sequence:

  1. TRCN0000003851