Construct: shRNA TRCN0000284792

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003678.4-323s21c1
DNA Barcode:
GATGTGGCAATAGAAATAGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
THOC5 (8563)
Gene Description:
THO complex 5
Transcript:
RefSeq NM_003678.4 (NON-CURRENT)
Match location:
Position 323 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000284792)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATGTGGCAATAGAAATAGAA
Hairpin Sequence:
5'-CCGG-GATGTGGCAATAGAAATAGAA-CTCGAG-TTCTATTTCTATTGCCACATC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATGTGGCAATAGAAATAGAACTCGAGTTCTATTTCTATTGCCACATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATGTGGCAATAGAAATAGAACTCGAGTTCTATTTCTATTGCCACATC-3'

Other clones with same target sequence:

  1. TRCN0000000114