Construct: shRNA TRCN0000284799

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006925.3-422s21c1
DNA Barcode:
AGTAGTCGCAGACCTCGAAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SRSF5 (6430)
Gene Description:
serine and arginine rich splicing factor 5
Transcript:
RefSeq NM_006925.3 (NON-CURRENT)
Match location:
Position 422 (CDS)
CRISPR Mechanism
CRISPR

Vector Information:

Vector Backbone:
pLKO_TRC005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000284799)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGTAGTCGCAGACCTCGAAAT
Hairpin Sequence:
5'-CCGG-AGTAGTCGCAGACCTCGAAAT-CTCGAG-ATTTCGAGGTCTGCGACTACT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGTAGTCGCAGACCTCGAAATCTCGAGATTTCGAGGTCTGCGACTACTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGTAGTCGCAGACCTCGAAATCTCGAGATTTCGAGGTCTGCGACTACT-3'

Other clones with same target sequence:

(none)