Construct: shRNA TRCN0000284843

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005744.3-1834s21c1
DNA Barcode:
GCTACCTTGAACGAGATATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ARIH1 (25820)
Gene Description:
ariadne RBR E3 ubiquitin protein ligase 1
Transcript:
RefSeq NM_005744.3 (NON-CURRENT)
Match location:
Position 1834 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000284843)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTACCTTGAACGAGATATTT
Hairpin Sequence:
5'-CCGG-GCTACCTTGAACGAGATATTT-CTCGAG-AAATATCTCGTTCAAGGTAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTACCTTGAACGAGATATTTCTCGAGAAATATCTCGTTCAAGGTAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTACCTTGAACGAGATATTTCTCGAGAAATATCTCGTTCAAGGTAGC-3'

Other clones with same target sequence:

  1. TRCN0000007502
  2. TRCN0000294688