Construct: shRNA TRCN0000284905

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_016323.2-2828s21c1
DNA Barcode:
GCGACGAAGACATTATCAAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
HERC5 (51191)
Gene Description:
HECT and RLD domain containing E3 ubiquitin protein ligase 5
Transcript:
RefSeq NM_016323.2 (NON-CURRENT)
Match location:
Position 2828 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000284905)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCGACGAAGACATTATCAAAT
Hairpin Sequence:
5'-CCGG-GCGACGAAGACATTATCAAAT-CTCGAG-ATTTGATAATGTCTTCGTCGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCGACGAAGACATTATCAAATCTCGAGATTTGATAATGTCTTCGTCGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCGACGAAGACATTATCAAATCTCGAGATTTGATAATGTCTTCGTCGC-3'

Other clones with same target sequence:

  1. TRCN0000004171