Construct: shRNA TRCN0000284952

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003350.2-378s21c1
DNA Barcode:
TCAAGAGCTAAGACGTCTAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
UBE2V2 (7336)
Gene Description:
ubiquitin conjugating enzyme E2 V2
Transcript:
RefSeq NM_003350.2 (NON-CURRENT)
Match location:
Position 378 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000284952)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCAAGAGCTAAGACGTCTAAT
Hairpin Sequence:
5'-CCGG-TCAAGAGCTAAGACGTCTAAT-CTCGAG-ATTAGACGTCTTAGCTCTTGA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCAAGAGCTAAGACGTCTAATCTCGAGATTAGACGTCTTAGCTCTTGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCAAGAGCTAAGACGTCTAATCTCGAGATTAGACGTCTTAGCTCTTGA-3'

Other clones with same target sequence:

(none)