Construct: shRNA TRCN0000285029

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_021809.5-281s21c1
DNA Barcode:
GCTGTACTTGCACCGCTACAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TGIF2 (60436)
Gene Description:
TGFB induced factor homeobox 2
Transcript:
RefSeq NM_021809.5 (NON-CURRENT)
Match location:
Position 281 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000285029)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGTACTTGCACCGCTACAA
Hairpin Sequence:
5'-CCGG-GCTGTACTTGCACCGCTACAA-CTCGAG-TTGTAGCGGTGCAAGTACAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGTACTTGCACCGCTACAACTCGAGTTGTAGCGGTGCAAGTACAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGTACTTGCACCGCTACAACTCGAGTTGTAGCGGTGCAAGTACAGC-3'

Other clones with same target sequence:

(none)