Construct: shRNA TRCN0000285151

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_138473.2-589s21c1
DNA Barcode:
ACCTGGAGTGATGCCTAATAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SP1 (6667)
Gene Description:
Sp1 transcription factor
Transcript:
RefSeq NM_138473.2 (NON-CURRENT)
Match location:
Position 589 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000285151)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACCTGGAGTGATGCCTAATAT
Hairpin Sequence:
5'-CCGG-ACCTGGAGTGATGCCTAATAT-CTCGAG-ATATTAGGCATCACTCCAGGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACCTGGAGTGATGCCTAATATCTCGAGATATTAGGCATCACTCCAGGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACCTGGAGTGATGCCTAATATCTCGAGATATTAGGCATCACTCCAGGT-3'

Other clones with same target sequence:

(none)