Construct: shRNA TRCN0000285244

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008029.2-3715s21c1
DNA Barcode:
CAGCCTGGCAGCCAGATATTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Flt4 (14257)
Gene Description:
FMS-like tyrosine kinase 4
Transcript:
RefSeq NM_008029.2 (NON-CURRENT)
Match location:
Position 3715 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000285244)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAGCCTGGCAGCCAGATATTA
Hairpin Sequence:
5'-CCGG-CAGCCTGGCAGCCAGATATTA-CTCGAG-TAATATCTGGCTGCCAGGCTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAGCCTGGCAGCCAGATATTACTCGAGTAATATCTGGCTGCCAGGCTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAGCCTGGCAGCCAGATATTACTCGAGTAATATCTGGCTGCCAGGCTG-3'

Other clones with same target sequence:

(none)