Construct: shRNA TRCN0000285285

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_139118.1-1021s21c1
DNA Barcode:
GCTCCTGACAACATCATTAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
YY1AP1 (55249)
Gene Description:
YY1 associated protein 1
Transcript:
RefSeq NM_139118.1 (NON-CURRENT)
Match location:
Position 1021 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000285285)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTCCTGACAACATCATTAAA
Hairpin Sequence:
5'-CCGG-GCTCCTGACAACATCATTAAA-CTCGAG-TTTAATGATGTTGTCAGGAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTCCTGACAACATCATTAAACTCGAGTTTAATGATGTTGTCAGGAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTCCTGACAACATCATTAAACTCGAGTTTAATGATGTTGTCAGGAGC-3'

Other clones with same target sequence:

  1. TRCN0000134438