Construct: shRNA TRCN0000285287

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018840.2-248s21c1
DNA Barcode:
CAAAGTCTCCGTCTGGAGTAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RAB5IF (55969)
Gene Description:
RAB5 interacting factor
Transcript:
RefSeq NM_018840.2 (NON-CURRENT)
Match location:
Position 248 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000285287)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAAAGTCTCCGTCTGGAGTAA
Hairpin Sequence:
5'-CCGG-CAAAGTCTCCGTCTGGAGTAA-CTCGAG-TTACTCCAGACGGAGACTTTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAAAGTCTCCGTCTGGAGTAACTCGAGTTACTCCAGACGGAGACTTTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAAAGTCTCCGTCTGGAGTAACTCGAGTTACTCCAGACGGAGACTTTG-3'

Other clones with same target sequence:

  1. TRCN0000155433