Construct: shRNA TRCN0000285295

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_020336.2-2404s21c1
DNA Barcode:
GTCGCACCAATAGTGGTATTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RALGAPB (57148)
Gene Description:
Ral GTPase activating protein non-catalytic beta subunit
Transcript:
RefSeq NM_020336.2 (NON-CURRENT)
Match location:
Position 2404 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000285295)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTCGCACCAATAGTGGTATTA
Hairpin Sequence:
5'-CCGG-GTCGCACCAATAGTGGTATTA-CTCGAG-TAATACCACTATTGGTGCGAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTCGCACCAATAGTGGTATTACTCGAGTAATACCACTATTGGTGCGACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTCGCACCAATAGTGGTATTACTCGAGTAATACCACTATTGGTGCGAC-3'

Other clones with same target sequence:

(none)